Optimization of Extraction of Triterpense from Geum japonicum using Response Surface Methodology

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China. _________________________________________________________________________________________________________________________ ABSTRACT This study aims to determine the best extraction conditions and find out the effects of solvent type (ethanol, aceton, and ionic liquid (1-butyl-3-methylimidazolium bromide)[BMIM]Br), extraction time (20–60min), and microwave power (200–600w) on extraction rate and triterpenes (TTP) of Geum japonicum. The three parameters, type solvents, extraction time and microwave power, were optimized using the Boxe Behnken design (BBD) with a quadratic regression model built by using response surface methodology (RSM). The experiments were carried out according to 17 runs with 3 variables and 3 levels for the optimization. The extracts were analyzed by spectrophotometeric methods for the (TTP). The optimal extraction conditions were determined as follows: (i) [BMIM]Br) solvents, microwave power 599.99 w, extraction time 60 min for the best extraction rate 34.1 % and TTP 3.9 mg/g. (ii) ethanol solvents, microwave power 235.77 w, extraction time 37.73 min for the extraction rate 19.8 % and best TTP 33.25 mg/g. This results showed that [BMIM][Br] solvent are more efficient in the extraction of extraction rate compare with ethanol and aceton solvents. While ethanol solvent are more efficient in the extraction of TTP compare with [BMIM][Br] and aceton solvents.